This summer we’re sponsoring high school interns in stem cell labs throughout California. We asked those students to contribute to our Instagram photos and YouTube videos about life in the lab, and write about their experiences.
Ariana Gonzalez did a stem cell research internship this summer in the laboratory of Keigo Machida at the University of Southern California.
|Ariana Gonzalez pipetting in the lab. She submitted this photo to our #CIRMStemCellLab Instagram feed|
During the summer I worked on a research project that involved silencing certain genes, knock down certain epigenetic regulators with lentiviral shRNA, this will be known as loss of function. This was used to analyze by how much the Nanog level increased or decreased. The desired effects were that Nanog levels would decrease since this would mean that gene is key in order for there to get a step closer on finding what genes can be silenced so that Nanog, which helps stem cells regenerate can be decreased in tumor imitating cells and eventually it will lead to a cure for liver cancer. The regulators include: histone de-acetylase, DNMT1, MLL1, ESET, Dot1, and HDAC1.
After this internship I have learned a lot about stem cells. First of all I learned from where they come from, which is from the Fertilization Clinics, where couples do in vitro fertilization. I also learned that in order for them to grow they need a feeder layer. Another thing was that they can divide in big numbers and therefore need to be passage every certain days, about 3-4 days, or else they start dying off since then they will grow in big numbers and lack resources.
I enjoyed everything from this internship. I liked to opportunity I got to do something like this and to be exposed to actual labatory work in a lab. I feel that it was a great opportunity to find out if research is something that you want to look into. My family thinks that this was a great opportunity because it exposes us to materials and things that we had never seen before and that other high school kids do not get an opportunity. It also helps us get exposed to things that are happening in the real world, things that not only affect one person, but a large population in the world.
I think that after this experience I do want to pursue a career in research.
Ariana submitted these videos about her experience: